TB-500: Research Overview
TB-500 is a synthetic peptide representing the active region of Thymosin Beta-4 (Tβ4), a naturally occurring 43-amino acid peptide found in virtually all human and animal cells. The "500" designation refers to a specific molecular weight fraction.
Peptide Profile
| Property | Details |
|---|---|
| Full Name | Thymosin Beta-4 Fragment |
| Related To | Thymosin Beta-4 (Tβ4) |
| Active Region | LKKTETQ sequence |
| Molecular Weight | ~4,963 g/mol |
| Purity | 99%+ (HPLC verified) |
| Form | Lyophilized powder |
Background
Thymosin Beta-4 was originally isolated from calf thymus tissue in the 1960s. It's found throughout the body and plays roles in:
TB-500 contains the key active sequence responsible for many of Tβ4's biological activities.
The LKKTETQ Sequence
The core active region of TB-500 is the LKKTETQ amino acid sequence, which has been identified as crucial for:
Research Applications
Tissue Research
Cell Biology
Comparative Studies
TB-500 is often studied alongside:
Key Research Areas
Actin Regulation
TB-500's interaction with G-actin (globular actin) has been extensively studied. Understanding this interaction helps researchers investigate:
Angiogenesis Research
Studies have examined TB-500's relationship to:
Tissue Regeneration Models
In-vitro and ex-vivo studies have explored:
Reconstitution Protocol
Standard Volumes
| Vial Size | BAC Water | Concentration |
|---|---|---|
| 2mg | 1ml | 2,000mcg/ml |
| 5mg | 2ml | 2,500mcg/ml |
| 5mg | 2.5ml | 2,000mcg/ml |
Procedure
Storage Guidelines
Lyophilized
| Storage | Temperature | Duration |
|---|---|---|
| Long-term | -20°C | 2+ years |
| Short-term | 2-8°C | 6-12 months |
Reconstituted
Physical Properties
Appearance
Lyophilized:
Reconstituted:
Solubility
TB-500 is highly water-soluble, dissolving readily in:
Handling Considerations
Research Dosing Reference
Common research concentrations vary by application:
| Application Type | Concentration Range |
|---|---|
| Cell culture | 1-100 ng/ml |
| Tissue studies | Variable |
| Binding assays | Research-dependent |
*Concentrations depend on specific research protocols and should be determined by the researcher.*
Quality Verification
Before use, verify:
Conclusion
TB-500 provides researchers with a tool to study the biological activities associated with Thymosin Beta-4. Its well-characterized structure and stability make it suitable for various laboratory applications in tissue and cell biology research.
*TB-500 is for laboratory research purposes only and is not approved for human or veterinary use.*
Frequently Asked Questions
What is the difference between TB-500 and Thymosin Beta-4?
TB-500 is a synthetic peptide fragment containing the active region of the full 43-amino acid Thymosin Beta-4 protein. TB-500 focuses on the key LKKTETQ sequence responsible for many biological activities.
How should TB-500 be reconstituted?
Add bacteriostatic water slowly along the vial wall, not directly onto the powder. Let it dissolve naturally for 5-10 minutes, then gently swirl if needed. Never shake. The solution should be clear and colorless.
Can TB-500 and BPC-157 be used in the same research?
Yes, many researchers study these peptides both independently and in combination. They have different mechanisms and research applications, making comparative and combined studies valuable.
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